Methods in molecular biophysics : structure, dynamics, function / Nathan R. Zaccai, Igor N. Serdyuk, Joseph Zaccai

Igor N. Serdyuk's name appears first in the previous edition

Includes bibliographical references and index

Macromolecules in their environment -- Macromolecules as physical particles -- Understanding macromolecular structures -- Mass and charge -- Structure function studies -- Thermodynamic stability and interactions -- Differential scanning calorimetry -- Isothermal titration calorimetry -- Surface plasmon resonance and interferometry-based biosensors -- Biological macromolecules as hydrodynamic particles -- Analytical ultracentrifugation -- Fluorescence depolarization -- Dynamic light scattering and fluorescence correlation spectroscopy -- Visible and IR absorption spectroscopy -- Two-dimensional IR spectroscopy -- Raman scattering spectroscopy -- Optical activity and circular dichronism -- Light microscopy -- Single molecule manipulation and atomic force microscopy -- Fluorescence microscopy -- The macromolecule as a radiation scattering particle -- Small-angle scattering and reflectometry -- X-ray and neutron macromolecular crystallography -- Electron microscopy -- Three-dimensional reconstruction from two-dimensional images -- Energy and time calculations -- Neutron spectroscopy -- Distances and angles from frequencies -- Experimental techniques -- Structure and dynamics studies -- Radiology and positron emission tomography -- Ultrasound imaging -- Magnetic resonance imaging

Current techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field. --Amazon.co.uk