000			04115cam a2200277 i 4500
999			_c8301 _d8301
001			341621
003			0000000000
005			20190111110741.0
008			150922s2016 njua b 001 0 eng
020			_a9781118175866
040			_erda
050			_aQP 519.9 _b.F673 2016
245	0	0	_aFluorescent analogs of biomolecular building blocks : _bdesign and applications / _cedited by Prof. Dr. Marcus Wilhelmsson, Department of Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, Prof. Dr. Yitzhak Tor, Department of Chemistry and Biochemistry, University of California, San Diego.
260			_aHoboken, New Jersey : _bWiley, _c[2016].
264		1	_aHoboken, New Jersey : _bWiley, _c[2016].
300			_axxii, 418 pages : _billustrations (some color) ; _c25 cm.
336			_atext _2rdacontent
337			_aunmediated _2rdamedia
338			_avolume _2rdacarrier
504			_aIncludes bibliographical references and index.
505	0		_aCover; Title Page; Copyright; Contents; List of Contributors; Preface; Chapter 1 Fluorescence Spectroscopy; 1.1 Fundamentals of Fluorescence Spectroscopy; 1.2 Common Fluorescence Spectroscopy Techniques; 1.2.1 Steady-State Fluorescence Spectroscopy; 1.2.2 Time-Resolved Fluorescence Spectroscopy; 1.2.3 Fluorescence Anisotropy; 1.2.4 Resonance Energy Transfer and Quenching; 1.2.5 Fluorescence Microscopy and Single Molecule Spectroscopy; 1.2.6 Fluorescence-Based in vivo Imaging; 1.3 Summary and Perspective; References Chapter 2 Naturally Occurring and Synthetic Fluorescent Biomolecular Building Blocks2.1 Introduction; 2.2 Naturally Occurring Emissive Biomolecular Building Blocks; 2.3 Synthetic Fluorescent Analogs of Biomolecular Building Blocks; 2.3.1 Synthetic Emissive Analogs of Membranes Constituents; 2.3.2 Synthetic Emissive Analogs of Amino Acids; 2.3.3 Synthetic Emissive Analogs of Nucleosides; 2.4 Summary and Perspective; References; Chapter 3 Polarized Spectroscopy with Fluorescent Biomolecular Building Blocks; 3.1 Transition Moments; 3.2 Linear Dichroism; 3.3 Magnetic Circular Dichroism 3.4 Forster Resonance Energy Transfer (FRET)3.5 Fluorescence Anisotropy; 3.6 Fluorescent Nucleobases; 3.7 Fluorescent Peptide Chromophores; 3.8 Site-Specific Linear Dichroism (SSLD); 3.9 Single-Molecule Fluorescence Resonance Energy Transfer (smFRET); 3.10 Single-Molecule Fluorescence-Detected Linear Dichroism (smFLD); References; Chapter 4 Fluorescent Proteins: The Show Must go on!; 4.1 Introduction; 4.2 Historical Survey; 4.3 Photophysical Properties; 4.3.1 Absorption Properties and Color Hue Modification; 4.3.2 Chromophore Formation; 4.3.3 Fluorescence Color and Dynamics 4.3.4 Directional Properties along with Optical Transitions4.3.5 Energy Transfer and Energy Migration; 4.4 Photochemical Reactions; 4.4.1 Excited-state Proton Transfer (ESPT); 4.4.2 Isomerization Reactions: Reversible Photoswitching; 4.4.3 Photoconversion: Irreversible Bond Rupture; 4.4.4 Other Photochemical Reactions; 4.5 Ion Sensitivity; 4.5.1 Ground-State Equilibria of Protonation States; 4.5.2 Quenching by Small Ions; 4.6 Relation Microscopy-Spectroscopy for Fluorescent Proteins; 4.6.1 Brightness Alteration from Cuvette to Microscopic Experiments; 4.6.2 Lessons from Microspectrometry 4.6.3 Tools for Advanced Microscopic Techniques4.7 Prospects and Outlook; Acknowledgments; References; Chapter 5 Design and Application of Autofluorescent Proteins by Biological Incorporation of Intrinsically Fluorescent Noncanonical Amino Acids; 5.1 Introduction; 5.2 Design and Synthesis of Fluorescent Building Blocks in Proteins; 5.2.1 Extrinsic Fluorescent Labels; 5.2.2 Intrinsic Fluorescent Labels; 5.2.3 Extrinsic Labels Chemically Ligated using Cycloaddition Chemistry; 5.2.4 Modification of the Genetic Code to Incorporate ncAAs; 5.3 Application of Fluorescent Building Blocks in Proteins.
650		0	_aFluorescence spectroscopy. _2sears _928470
700	1		_aWilhelmsson, Marcus, _d1972- _eeditor. _928471
700	1		_aTor, Yitzhak, _eeditor. _928472
942			_2lcc _cGS
984			_a066739 _blac/sms